https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38049 Wed 28 Jul 2021 16:00:45 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45206 Cyp11a1-Cre to specifically ablate AR from the mouse adrenal cortex. Results show that AR-signaling is dispensable for adrenal gland development in females, and for X-zone regression during pregnancy, but is required to suppress elevation of corticosterone levels post-partum. Additionally, following disruption to adrenal AR, aberrant spindle cell development is observed in young adult females. These results demonstrate sexually dimorphic regulation of the adrenal X-zone by AR and point to dysfunctional adrenal androgen signaling as a possible mechanism in the early development of adrenal spindle cell hyperplasia.]]> Wed 26 Oct 2022 17:41:19 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44983 in vitro upregulated steroidogenic genes but did not fully induce LC differentiation. In vivo, no difference in LC-regeneration was noted between Sham and hAd-PSC-transplanted rats. Interestingly, Cyp17a1 expression increased in hAd-PSC-transplanted testes compared to intact vehicle controls and the luteinising hormone/testosterone ratio returned to Vehicle control levels which was not the case in EDS + Sham animals. Notably, hAd-PSCs were undetectable one-month after transplantation suggesting this effect is likely mediated via paracrine mechanisms during the initial stages of regeneration; either directly by interacting with regenerating LCs, or through indirect interactions with trophic macrophages.]]> Thu 27 Oct 2022 09:17:40 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34486 Thu 27 Jan 2022 15:56:27 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48411 Thu 16 Mar 2023 14:04:16 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47486 Mon 23 Jan 2023 11:47:26 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45503 Fri 28 Oct 2022 16:07:11 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52812 in vivo and validated which of these are likely to arise primarily from Sertoli cells based on relevant mouse testis RNASeq datasets. We used a different, but complementary, approach to identify Sertoli cell-enriched proteins in human TIF, taking advantage of high-quality human testis genomic, proteomic and immunohistochemical datasets. We identified a total of 47 and 40 Sertoli cell-enriched proteins in mouse and human TIF, respectively, including 15 proteins that are conserved in both species. Proteins with potential roles in angiogenesis, the regulation of Leydig cells or steroidogenesis, and immune cell regulation were identified. The data suggests that some of these proteins are secreted, but that Sertoli cells also deposit specific proteins into TIF via the release of extracellular vesicles. In conclusion, we have identified novel Sertoli cell-enriched proteins in TIF that are candidates for regulating somatic cell-cell communication and testis function.]]> Fri 27 Oct 2023 15:56:20 AEDT ]]>